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Viral Hemorrhagic Septicemia virus (VHSv) Infection in Lake Erie Yellow Perch, Perca flavescens, and its Effect on Feeding.

作者:Gregory M. Bergquist,
畢業學校:Case Western Reserve University
出版單位:Case Western Reserve University / OhioLINK
核准日期:2012-08-24
類型:Electronic Thesis or Dissertation
權限:restricted; full text unavailable until 2013-08-17.This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable cop....

英文摘要

Following the recent detection of VHSv in the Great Lakes I conducted a study on VHSv in Lake Erie yellow perch. A newly developed RT-PCR and nested-PCR assay was tested on yellow perch blood samples collected in 2008 and compared to the cell culture method of testing. I found that the RT-PCR assay coupled with the nested PCR was more sensitive in detecting VHSv in yellow perch than the cell culture method used by testing laboratories. The effects of VHSv infection on yellow perch feeding were investigated by analysis of growth patterns in yellow perch otoliths. Periods of slow growth in the early portion of the growing season were significantly more frequent in peak-VHSv years than pre-VHSv years. Lastly, phylogenetic analysis of nucleocapsid gene sequences from VHSv showed sequence differences from Great Lakes isolates were associated with geographic location and not host species or time of isolation.


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