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以基因轉殖植物分析水稻OsMADS14基因功能

Functional analysis of rice OsMADS14 in transgenic plants

作者:陳秋帆
畢業學校:輔英科技大學
出版單位:輔英科技大學
核准日期:2012-08-16
類型:Electronic Thesis or Dissertation
權限:Copyright information available at source archive--Fooyin University....

英文摘要

Oncidium is one of the most important orchids used for cut flowers and potted plants in Taiwan. The creation of new cultivars with novel traits is important for Oncidium to enhance the competitiveness of orchid industry. However, traditional breeding processes are limited by the long life cycle and self-incompatibility. The objectives of this study intend to analyze rice AP1-like gene OsMADS14 in Arabidopsis and Oncidium through genetic transformation. In this study, the rice gene OsMADS14 driven by maize ubiqutin promoter was introduced into Arabidopsis and Oncidium Gower Ramsey mediated by Agrobacterium tumefaciens. Twenty one independent ubiquitin::OsMADS14 transgenic Arabidopsis plants were produced, confirmed by PCR and RT-PCR analysis. The ectopic expression of OsMADS14 in transgenic Arabidopsis plants showed dwarf, early flowering and terminal flowers. In addition, the OsMADS14 gene were introduced into Oncidium Gower Ramsey using Agrobacterium tumefaciens-mediated transformation, protocorm-like bodies ( PLBs ) of Oncidium were used as explants materials for genetic transformation and selected on medium containing 5ppm hygromycin. The resistant transgenic Oncidium were analyzed by PCR, RT-PCR and histochemical GUS assay, indicating the transgene integrated into the genome. The resistant transgenic Oncidium showed leave bending phenotype. The results of this study suggest that the rice OsMADS14 gene could be applied early-flowering and dwarf traits to ornamental flowers.


召集委員 - 黃文理

委員 - 朱木貴

指導教授 - 李佩芳


 

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